جداسازی و شناسایی باکتری بورخولدریا سپاسیه از ترشحات تنفسی بیماران سیستیک فیبروزیس

Background and Aim: Cystic fibrosis is a common hereditary and autosomal disorder. One of the factors in cystic fibrosis is Burkholderia cepacia which can be transmitted through the sharing of admitted patients with hospitalized patients. Purpose of this study, was isolation and identification of Burkholderia cepacia from respiratory secretions from Masih Daneshvari Hospital cystic fibrosis patient. Materials and Methods: In this study, during 8 months from 2011 till 2012, 100 cases of pulmonary secretions were collected in Masih Daneshvari hospital patients. The specimens were cultured on both MacConkey agar and Burkholderia cepacia selective agar (BCSA) medium. After an incubation of 48 hours at 35°C, suspected colonies were isolated and by using of phenotypic and biochemical tests like TSI agar, simmons citrate medium, indol and motility, MRVP, oxidation - fermentation (OF), arginine- ornithine - lysine decarboxylase test, Dnase test, and polymyxin resistance were identified. Results: Using BCSA medium and biochemical tests led to isolation and identification of five Burkholderia cepacia strains from 100 respiratory samples. However, using Mac Conkey no Burkholderia cepacia was detected. Conclusion: Our results clearly indicate the use of BCSA medium as a specific medium for isolation of Burkholderia cepacia in cystic fibrosis patients. So, presence of 5% Burkholderia cepacia in cystic fibrosis infections suggests detecting this bacterium, in a specific and sensitive culture medium.