تأثیر استرونشیوم برمیزان رشد و تکوین جنین‌های دو سلولی متوقف شده موش سوری

Introduction: Decrease in the rate of the growth and development embryos and their arrest in a certain developmental step e.g. two- Celll block, could be the reason of infertility in some couples reffering to fertility and infertility center. The aim of this study is the evaluation of the effect of strontium on growth and development of two-Celll arrested NMRI mouse embryos. Methods: Following superovulation, female mice were coupled with males and mice with positive vaginal plaque were killed 48 hours after HCG injection by cervical dislocation. Subsequently, two-Celll embryos were collected in RPMI(Roswell Park Memorial Institute) and were divided into 3 groups. The 1st group (control) were washed and incubated without any exposure in order to be cultured in M16 medium. The 2nd and 3rd groups were exposed to 4°C temperature for 24 hours. The 2nd group was incubated immediately, while the 3rd group was exposed to 10 mM strontium for 5 minutes before incubation. Results: The data analysis by one-way Anova showed that exposure to 4°C temperature caused a significant increase in degenerated embryos and a significant difference between groups(P=0.006). Our results showed that the mean percent of blastocyst formation(33.4%) and cleavage rate(45.2%) was decreased in 2nd group in comparison to 1st group(%77.7 versus 90.9%). Use of strontium in 3rd group didn’t have any effect on the cleavage rate(45.2% versus 45.5% in 3rd and 2nd groups, respectively), while the blastocyst formation rate was significantly increased in 3rd group(61%) comparing 2nd group(P=0.019). Conclusion: Developmental rate of arrested two-Celll mice embryos was significantly increased by strontium.