بررسی همبستگی پلی مورفیسم تک نوکلئوتید rs11614913 ژن mir196a2 با استعداد ابتلا به سرطان پستان در جمعیت جنوب ایران

Breast cancer is the most common cancer worldwide and is the second leading cause of death in women after lung cancer. Micro RNAs (miRNAs) are among endogenous factors which are involved in many types of cancers, including breast cancer. Single nucleotide polymorphism (SNP) in the miRNAs, might change their biological activities such as their effects on oncogenes and tumor suppressor genes. Therefore some of miRNA’s SNPs are associated with the risk of different types of cancer, including breast cancer. The aim of this study was to evaluate the correlation between SNP rs11614913 in mir196a2 and the risk of breast cancer. SNP rs11614913 in hsa-miR-196a2 analyzed in 100 breast cancer cases and 100 controls in women living in southern Iran. Polymorphism was identified by the PCR-RFLP method. The PCR product was digested with TaaI restriction enzyme which produced two bands with the length of 187bp and 196bp. The data from this case –control study were analyzed using odds ratios (ORs) with 95% confidence intervals (CIs) to reveal the associations of SNPs in miRNAs with breast cancer susceptibility. The C allele frequencies in patients and controls for miR-196a2 were 0.74 and 0.75, respectively. The genotype frequencies of TT, TC, and CC were 0.05, 0.42, and 0.53 for the patients and 0.06, 0.38, and 0.56 for the controls, respectively. Statistical analysis showed that the genetic frequencies for this SNP were in Hardy-Weinberg equilibrium (p>0.05). The results of this study indicated no significant association between SNP rs11614913 in mir196a2 and the risk of breast cancer.