بررسی رابطه ژن‌های فسفولیپاز C با پاتوژنیسیته مایکوباکتریوم توبرکلوزیس سویه بیجینگ وسویه غیربیجینگ

Abstract Background: The Beijing strain of Mycobacterium tuberculosis is responsible for more than 25% of cases of Tuberculosis in the world, it has a high transmission potential and there is a significant correlation between Beijing strain and multidrug resistance. In this study we compared the presence of Phospholipase C genes in Beijing and Nonbeijing strains of Mycobacterium tuberculosis. Methods: CTAB method was used to extract DNA from positive culture specimens from patients with pulmonary tuberculosis. Spoligotyping was used to identify Beijing from non-Beijing strains. Presence of Phospholipase C genes was ascertained by using PCR. Result: Of 200 specimens, 19 were Beijing strain, (9.5%) and 181 non Beijing strains, (90.5%). In Beijing strains, 16 specimens, (84.2%)were positive for plcA,17 (89.5%) were positive for plcB and 17 (89.5%) were positive for plcC genes and in non Beijing strains 17 specimens, (9.4% ), were positive for plcA, 18(9.9%), for plcB and 18(9.9%) for plcC. Conclusion: Majority of Beijing strains have phospholipase C genes that may be responsible for the virulence of this strain. Keywords: Phospholipase C, MDR, Beijing, Mycobacterium tuberculosis