بررسی اثر سیتوتوکسیک عصاره اتانولی برگ گیاه پسته وحشی (Pistacia Khinjuk) بر دو رده سلول سرطانی Hela و MCF-7

Background and Objectives: Cancer is the most important cause of death worldwide. Natural products and derivatives of medicinal plants can play an important role to cure cancer. The aim of this study was to evaluate the cytotoxic effect of ethanolic extracts of Pistacia Khinjuk leaf on Hela and MCF-7 cell lines. Materials and Methods: In this laboratory study, ethanolic extract of Pistacia Khinjuk leaves was prepared by using maceration method and its cytotoxic effect on Hela and MCF-7 cell lines was investigated. Hela and MCF-7 cell lines were treated with various concentrations of extract (0.156-10.0 mg/ml) for 72 hours and growth inhibition was assayed using MTT )Methy Thiazol Tetrazolium) test. The optical density of the coloured solution was quantified at 570 nm wavelengths by an ELISA Reader. Data were analyzed using one-way ANOVA and Dunnett's post-test. Results: The results showed that different concentrations of ethanolic extract significantly reduced the growth of Hela and MCF-7 cell lines compared to the control group after 72 hours (P< 0.05). The highest percentages of growth inhibition at concentration of 0.156 mg/ml were 81.33% and 76.76% and the IC50 values were 2.41 and 2.40 mg/ml for Hela and MCF-7 cell lines, respectively. Conclusion: The findings of the present study showed that the ethanolic extract of Pistacia Khinjuk leaf possessed inhibitory effect on Hela and MCF-7 cell lines. In order to find the underlying mechanism of this activity, further research should be carried out. Key words: Cytotoxicity, Ethanolic extract, Pistacia Khinjuk, Hela and MCF-7 cell lines Funding: This research was funded by Islamic Azad University, Qaemshahr Branch. Conflict of interest: None declared. Ethical approval: This article does not need permission from the Ethics Committee because the information in this article was derived from a non-animal research. How to cite this article: Seyedalipour B, Pourakbar E, Taravati A. The Cytotoxic Effect of Ethanolic Extract of Pistacia Khinjuk Leaf Aganist HeLa and MCF-7 cancerous Cell Lines. J RafsanjanUniv Med Sci 2016; 14(11): 939-52. [Farsi]