بررسی اثرات آنتی‌اکسیدانی و اندازه‌گیری ترکیبات فنلی عصاره‌های جزئی قارچ دارویی Phellinus torulosus از ایران

  Background and Objectives: Phellinu s Quel is a large and widely distributed genus of the family Hymenochaetaceae under the class of Basidiomycetes with more than 359 species. The Phellinu s species have anticancer, antioxidant and antibacterial effects . Hitherto, 12 Phellinus species have been reported from the north of Iran. Due to their great medicinal effects and lack of investigation on Iranian species , this study was performed to investigate the host dispersal behaviour, macro- and micro-morphological properties of the species, and antioxidant activities. using the total methanol extract and it’s fractional extracts (chloroform, butanol and water extracts).   Materials and Methods: In this experimental study, the macro- and micro-morphological properties of the species were determined using the total methanol extract and it’s fractional extracts (chloroform, butanol and water extracts). The antioxidant assays were then performed. The radical scavenging capacity of fractional extracts of this fungus were evaluated by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and Ferric reducing-antioxidant power (FRAP) assays. Also, the total phenol content (TPC) of each extract was measured using Folin-Ciocalteau method.   Results: The results indicated that the total methanol extract showed a significant antioxidant activity. Further fractionation indicated that the butanol fraction had a stronger activity than the total methanolic extract in both antioxidant assays. The effective concentrations of the different extracts (total methanol, chloroform, butanol and water), for scavenging of 50% of generated radicals (IC50), was 30.87±2.07, 87.22±2.56, 11.98±0.32 and 229.67±0.94 µg/ml, respectively.   Conclusion: The results showed that different extracts, especially butanol extract have high antioxidant activities which indicate the presence of active components in this fraction. Considering these great antioxidant effects and its low expense , it is recommended to use this fungus in more clinical trial researches and drug manufacturing industry.   Key words : Phellinus, Antioxidant effects, Phenolic compounds, Medicinal mushroom, Native of Iran     Funding : This research was founded by Shaheed Beheshti University, Tehran, Iran.   Conflict of interest: None declared.   Ethical approval: None declared.     How to cite this article: Hokmollahi F, Rafati H, Riahi H, Hakimi MH, Heydari H, Haghirosadat F, et al. Investigation of the Antioxidant activity and determination of total phenol content of medicinal mushroom, Phellinus torulosus fractional extracts from Iran. J Rafsanjan Univ Med Sci 2011 57-69. [Farsi]