اندازه گیری میزان فعالیت سلولولیتیک دسته ای از آنزیم های میکربی و بررسی کیفی محصولات هیدرولیز آنزیماتیک سلولز

The cellulolytic activities of eighteen aerobic fungi, isolated from the microflora of Iran, with respect to Avecil were compared with the cellulolytic activity of Trichoderma reesei, CBS 383.78. The data indicated that the majority of the isolated microorganisms were capable of hydrolysing Avicel in the solid agar media to a better extent than Treesei. However, the hydrolylic activities of the same test microorganisms in the liquid media containing A vecil were less than the relevant activity of T'Reesei. Thin-layer chromatography analysis of the products formed by each fungi from whatman filter o paper (no. 1) at 50 C for two hours of hydrolysis, were mostly consisted of small-size oligosaccharides, while the corresponding products formed by Treesei have higher molecular weights.