اثر متیل جاسمونات بر میزان بیان ژن‌های کاتالاز، پرولین سنتتاز، بتایین آلدهید دهیدروژناز و SOS1 در گیاه دارویی بابونه تحت تنش شوری

Chamomile (Tanacetum parthenium) is one of the most valuable medicinal plants and its dried flowers contain many terpenoids and flavonoids contributing to its medicinal properties. Salinity is one of the main limiting factors of plants growth in the world, especially in arid and semi-arid areas. Currently, biological methods use widely to contrast the soil salinity. To study of the effect of methyl jasmonate on effective gene expression under salt stress in chamomile, factorial experiment in a completely randomized design did in 3 replications. Salinity treatments at four levels (0, 50, 100 and 150 mM) and methyl jasmonate at four levels (0, 50, 100 and 150 µM) were applied. Sampling at four times (0, 24, 36 and 72 h) and the interaction between time and salinity and methyl jasmonate treatment were evaluated. In different concentrations of salinity, the results of studied genes expression showed that the highest expression of BADH, SOS, Catalase and P5CS were at 150, 100, 150 and 50 mM concentrations of salinity, respectively. The results of studied genes expression in different methyl jasmonate concentrations and different times showed that the expression of BADH and Catalase genes were increased with increasing concentrations of methyl jasmonate and sampling time. The highest amount of expression of P5CS and SOS genes at 150 µM methyl jasmonate concentration was observed after 24 and 36h. The results of the interaction between salinity, methyl jasmonate and sampling time showed that the highest levels of gene expression for all genes was in 100 µM methyl jasmonate and 100 mM NaCl during 36 hours and the lowest level of expression was  in the control treatment.