The Intriguing Life of Autophagosomes
نویسندگان
چکیده
Autophagosomes are double-membrane vesicles characteristic of macroautophagy, a degradative pathway for cytoplasmic material and organelles terminating in the lysosomal or vacuole compartment for mammals and yeast, respectively. This highly dynamic, multi-step process requires significant membrane reorganization events at different stages of the macroautophagic process. Such events include exchange and flow of lipids and proteins between membranes and vesicles (e.g., during initiation and growth of the phagophore), vesicular positioning and trafficking within the cell (e.g., autophagosome location and movement) and fusion of autophagosomes with the boundary membranes of the degradative compartment. Here, we review current knowledge on the contribution of different organelles to the formation of autophagosomes, their trafficking and fate within the cell. We will consider some of the unresolved questions related to the molecular mechanisms that regulate the "life and death" of the autophagosome.
منابع مشابه
The Source of Human Knowledge: Plato’s problem and Orwell’s problem
Chomsky cannot help wondering at the fact that we, despite so vast evidence, have little knowledge about the obvious evidence. A good example, I think, is the child’s way of first language acquisition. A great many researchers have studied various aspects of child language acquisition at different stages of the child’ life and have brought to light many details of language development. However,...
متن کاملThe Source of Human Knowledge: Plato’s problem and Orwell’s problem
Chomsky cannot help wondering at the fact that we, despite so vast evidence, have little knowledge about the obvious evidence. A good example, I think, is the child’s way of first language acquisition. A great many researchers have studied various aspects of child language acquisition at different stages of the child’ life and have brought to light many details of language development. However,...
متن کاملپیوند خون بند ناف
Interest in umbilical cord blood as an alternative source of hematopietic stem cells is growing rapidly. Umbilical cord blood offers the clinician a source of hematopoietic stem cells that are readily available and rarely contaminated by latent viruses. Moreover, the collection of umbilical cord blood poses no risk to the donor. There is no need for general anesthesia or blood replacement and t...
متن کاملThe role of autophagy in advanced glycation end product-induced proliferation and migration in rat vascular smooth muscle cells
Objective(s): To investigate the role of autophagy in advanced glycation end products (AGEs)-induced proliferation and migration in rat vascular smooth muscle cells (VSMCs).Materials and Methods: After culture, VSMCs were treated with 0, 1, 10, and 100 μg/ml concentrations of AGEs. Autophagy specific protein light chain 3 (LC3)-I/II was determined by western blotting, autophagosomes were observ...
متن کاملAccumulation of autophagosomes confers cytotoxicity
Autophagy comprises the processes of autophagosome synthesis and lysosomal degradation. In certain stress conditions, increased autophagosome synthesis may be associated with decreased lysosomal activity, which may result in reduced processing of the excessive autophagosomes by the rate-limiting lysosomal activity. Thus, the excessive autophagosomes in such situations may be largely unfused to ...
متن کامل