Replication of Avocado Sunblotch Viroid in the Cyanobacterium Nostoc Sp. PCC 7120
نویسندگان
چکیده
1Aix Marseille University, CNRS, UMR Chemistry Laboratory Bacterial 7283. 31 Chemin Joseph Aiguier 13009 Marseille Cedex 20, France 2Institute of Systematics, Evolution, Biodiversity ( ISyEB ), CNRS, MNHN , UPMC, EPHE, UPMC Sorbonne University, 57 rue Cuvier, PO Box 50, F-75005 Paris, France 3Team Enzymology of RNA, UR6, UPMC Sorbonne University, 75252 Paris Cedex 05 France 4Laboratory of Plant Biotechnology, Faculty of Sciences of Sfax, University of Sfax, BP 1171, 3000 Sfax, Tunisia
منابع مشابه
Hydrogenases in Nostoc sp. Strain PCC 73102, a Strain Lacking a Bidirectional Enzyme.
The present study was carried out in order to examine and characterize the bidirectional hydrogenase in the cyanobacterium Nostoc sp. strain PCC 73102. Southern hybridizations with the probes Av1 and Av3 (hoxY and hoxH, bidirectional hydrogenase small and large subunits, respectively) revealed the occurrence of corresponding sequences in Anabaena variabilis (control), Anabaena sp. strain PCC 71...
متن کاملHupW protease specifically required for processing of the catalytic subunit of the uptake hydrogenase in the cyanobacterium Nostoc sp. strain PCC 7120.
The maturation process of [NiFe] hydrogenases includes a proteolytic cleavage of the large subunit. We constructed a mutant of Nostoc strain PCC 7120 in which hupW, encoding a putative hydrogenase-specific protease, is inactivated. Our results indicate that the protein product of hupW selectively cleaves the uptake hydrogenase in this cyanobacterium.
متن کاملThe 5' end generated in the in vitro self-cleavage reaction of avocado sunblotch viroid RNAs is present in naturally occurring linear viroid molecules.
The 5' termini of linear forms of avocado sunblotch viroid (ASBVd) isolated from infected tissue have been determined by primer extension analyses. According to the different termini identified, four main populations of linear viroid molecules were found. One of them had the same 5' end as the one produced in the in vitro self-cleavage reaction of ASBVd dimeric transcripts mediated by a hammerh...
متن کاملSouth African Avocado Growers’ Association Yearbook 1986. 9:75-77 RECENT EXPERIENCE WITH THE USE OF SYNTHETIC DNA PROBES FOR THE DETECTION OF AVOCADO SUNBLOTCH VIROID
Recent experience using 23 oligonucleotides to diagnose avocado sunblotch viroid (ASBV) is described. In a number of field collected samples with sunblotch symptoms, the probe was unsuccessful in detecting the viroid. The sunblotch viroid varies greatly from branch to branch in a tree. Tree to tree variation is also common. However, the probe can be used with a reasonable measure of success to ...
متن کاملA chloroplast protein binds a viroid RNA in vivo and facilitates its hammerhead-mediated self-cleavage.
Viroids, small single-stranded circular RNAs (246-401 nucleotides), do not have mRNA capacity and must recruit host proteins to assist in the steps of their biological cycle. The nature of these cellular factors is poorly understood due to a lack of reliable experimental approaches. Here, to screen for host proteins interacting with viroid RNAs in vivo, we UV-irradiated avocado leaves infected ...
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