Acoustic Enrichment of Microvesicles in Plasma
نویسندگان
چکیده
We present for the first time an acoustofluidic method to enrich cell-derived extracellular vesicles (EV) from human plasma, enabling rapid access to a biological nano-compartments rich in biomarkers. The system was characterized using polystyrene nanoparticles. Human EVs from cell-free plasma were successfully enriched and analyzed using a FACS. The technique enables EV-recovery from small sample volumes, not possible with standard techniques. It also avoids the cumbersome standard method of hourlong ultracentrifugation steps and manual sample handling required to purify and enrich the 100 – 1000 nm large microvesicles.
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