Characterization of denitrification gene clusters of soil bacteria via a metagenomic approach.
نویسندگان
چکیده
We characterized operons encoding enzymes involved in denitrification, a nitrogen-cycling process involved in nitrogen losses and greenhouse gas emission, using a metagenomic approach which combines molecular screening and pyrosequencing. Screening of 77,000 clones from a soil metagenomic library led to the identification and the subsequent characterization of nine denitrification gene clusters.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 75 2 شماره
صفحات -
تاریخ انتشار 2009