TRE17/USP6 regulates ubiquitylation and trafficking of cargo proteins that enter cells by clathrin-independent endocytosis.

TRE 17 / USP 6 regulates ubiquitination and trafficking of cargo proteins that enter cells by clathrin - independent endocytosis

Discovery of new cargo proteins that enter cells through clathrin-independent endocytosis.

Clathrin-independent endocytosis (CIE) allows internalization of plasma membrane proteins lacking clathrin-targeting sequences, such as the major histocompatibility complex class I protein (MHCI), into cells. After internalization, vesicles containing MHCI fuse with transferrin-containing endosomes generated from clathrin-dependent endocytosis. In HeLa cells, MHCI is subsequently routed to late...

MARCH ubiquitin ligases alter the itinerary of clathrin-independent cargo from recycling to degradation

Following endocytosis, internalized plasma membrane proteins can be recycled back to the cell surface or trafficked to late endosomes/lysosomes for degradation. Here we report on the trafficking of multiple proteins that enter cells by clathrin-independent endocytosis (CIE) and determine that a set of proteins (CD44, CD98, and CD147) found primarily in recycling tubules largely failed to reach ...

Cargo Takes Control of Endocytosis

Clathrin-mediated endocytosis is a major route for diverse cargo to enter the cellular endocytic pathway. New findings by Puthenveedu and von Zastrow (2006) suggest that certain cargo proteins, such as G protein coupled receptors that bind to PDZ domains, regulate their own targeting to a subset of clathrin-coated pits. Thus, cargo proteins may cause functional specialization of vesicle traffic...

Microtubule-dependent endosomal sorting of clathrin-independent cargo by Hook1

Many plasma membrane (PM) proteins enter cells nonselectively through clathrin-independent endocytosis (CIE). Here, we present evidence that cytoplasmic sequences in three CIE cargo proteins-CD44, CD98, and CD147-were responsible for the rapid sorting of these proteins into endosomal tubules away from endosomes associated with early endosomal antigen 1 (EEA1). We found that Hook1, a microtubule...