The effect of viomycin on elongation steps in cell-free polypeptide synthesis.

that VMinhibits protein synthesis was first shown in intact Mycobacterium aviurrP and it is a ribosomal inhibitor since VM resistance of certain mutants of Mycobacterium smegmatis and Mycobacterium tuberculosis can be attributed to an alteration of ribosomes4~7). The characteristics of VMbinding to ribosomes have been studied. The drug reduced the amounts of [3H]dihydrostreptomycin bound to ribosomes of M. smegmatis and Escherichia coli suggesting an interaction between the binding sites of these antibiotics8). [14C]Tuberactinomycin O (TMO), VM derivative, bound to