Analysis of factors involved in plastid gene expression in the unicellular green alga Chlamydomonas reinhardtii

Synthesis of the D2 protein of photosystem II in Chlamydomonas is controlled by a high molecular mass complex containing the RNA stabilization factor Nac2 and the translational activator RBP40 23 3.2 Enrichment of native, high molecular weight ribonucleoprotein complexes from chloroplast by consecutive gel filtration steps 37 3.3 An intermolecular disulfide-based light switch for chloroplast psbD gene expression in Chlamydomonas reinhardtii 44 3.4 Cysteine modification of a specific repressor protein controls the translational status of nucleus-encoded LHCII mRNAs in Chlamydomonas 74 3.5 Chloroplast DnaJ-like proteins 3 and 4 (CDJ3/4) from Chlamydomonas reinhardtii contain redox-reactive Fe-S clusters and interact with stromal HSP70B 81 3.6 MRL1, a conserved pentatricopeptide repeat protein, is required for stabilization of rbcL mRNA in Chlamydomonas and Arabidopsis 95 4. Discussion 111 4.1 Binary set of factors for protein synthesis in the chloroplast of Chlamydomonas 111 4.2 Influence of post-translational changes on protein synthesis 115 4.3 Influence of organellar redox state on chloroplast translation activity 116 5. Appendix 120 5.1 List of publications 120 6. References 121 Danksagung 135 Ehrenwörtliche Versicherung / Erklärung 136 Abbreviations ADP adenosine diphosphate ATP adenosine triphosphate CDJ chloroplast DnaJ-like CES control of epistasy by synthesis CP chlorophyll binding protein CRP chloroplast RNA processing DNA deoxyribonucleic acid dsRNA double-stranded ribonucleic acid ER endoplasmic reticulum FAD flavin adenine dinucleotide HMW high molecular weight HMWC high molecular weight complex hnRNP K heterogeneous nuclear ribonucleoprotein K KH hnRNP K homology LDM low density membrane LHCII light harvesting complex of photosystem II mRNA messenger RNA NADP oxidized form of nicotinamide adenine dinucleotide phosphate NADPH reduced form of nicotinamide adenine dinucleotide phosphate NEP nucleus encoded plastid RNA polymerase OPR octatricopeptide repeat ORF open reading frame PEP plastid encoded plastid RNA polymerase pH negative decimal logarithm of proton activity PPR pentatricopeptide repeat PSI photosystem I PSII photosystem II PTM post-translational modification RB / RBP RNA binding protein RBD RNA binding domain RNA ribonucleic acid RNase ribonuclease RNP ribonucleoprotein ROS reactive oxygen species RRM RNA recognition motif rRNA ribosomal RNA Rubisco ribulose-1,5-biphosphat-carboxylase/oxygenase S Svedberg, sedimentation coefficient SD Shine-Dalgarno SDS-PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis SEC size exclusion chromatography ssDNA single-stranded DNA TPR tetratricopeptide repeat tRNA transfer RNA UV ultraviolet Gene abbreviations atpA gene encoding the  subunit of the CF 1 ATP synthase atpB gene encoding the  subunit of the CF 1 ATP synthase atpC gene encoding the  subunit of the CF 1 ATP synthase lhcb gene encoding …