Exogenous RNA interference exposes contrasting roles for sugar exudation in host-finding by plant pathogens.
نویسندگان
چکیده
Plant parasitic nematodes (PPN) locate host plants by following concentration gradients of root exudate chemicals in the soil. We present a simple method for RNA interference (RNAi)-induced knockdown of genes in tomato seedling roots, facilitating the study of root exudate composition, and PPN responses. Knockdown of sugar transporter genes, STP1 and STP2, in tomato seedlings triggered corresponding reductions of glucose and fructose, but not xylose, in collected root exudate. This corresponded directly with reduced infectivity and stylet thrusting of the promiscuous PPN Meloidogyne incognita, however we observed no impact on the infectivity or stylet thrusting of the selective Solanaceae PPN Globodera pallida. This approach can underpin future efforts to understand the early stages of plant-pathogen interactions in tomato and potentially other crop plants.
منابع مشابه
ExoRNAi exposes contrasting roles for sugar exudation in host - finding by 1 plant pathogens 2
متن کامل
ExoRNAi exposes contrasting roles for sugar exudation in host - finding by 1 plant pathogens 2 Neil
متن کامل
ExoRNAi exposes contrasting roles for sugar exudation in host - finding by 1 plant pathogens
متن کامل
ExoRNAi , a new tool to probe plant gene function exposes contrasting roles 1 for sugar exudation in host - finding by plant pathogens
12 Here we present a simple and rapid new method for RNAi-induced knockdown of genes in 13 tomato seedlings, through treatment with an aqueous solution of double-stranded RNA 14 (exoRNAi). The exoRNAi method is used to assess the involvement of tomato Sugar 15 Transporter Protein genes, stp1 and stp2 on the root exudation of glucose, fructose and 16 xylose; monosaccharide constituents of tomato...
متن کاملMultigene phylogeny and morphotaxonony of Septoria spp. from Iran along with a checklist of septoria-like taxa
The genus Septoria includes important plant pathogens with worldwide distribution, commonly associated with leaf spots and stem cankers of a broad range of plant hosts. In this study, eight isolates of Septoria were recovered from leaves with leaf spot on four herbaceous and woody plants from Gilan, Ardebil, East and West Azerbaijan provinces in north and northwest of Iran. The isolates were st...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- International journal for parasitology
دوره 46 8 شماره
صفحات -
تاریخ انتشار 2016