Molecular regulation of progesterone synthesis in the bovine corpus luteum

In bovine luteal cells, progesterone can directly affect its own synthesis by increasing the activity of 3β-HSD. The effect of progesterone on its own secretion coincides with increased expression of the genes for 3βHSD, StAR, and cytochrome P450scc. Therefore, progesterone regulates its own synthesis by affecting the activity of the enzymes that take part in luteal steroidogenesis, and also by affecting the expression of the genes for these enzymes. The aims of this study were: (a) to determine whether progesterone affects the expression of the gene for its own receptor, thereby affecting its own synthesis; and (b) to determine whether oxytocin and noradrenaline affect the expression of the genes for the oxytocin receptor (OT-R), the progesterone receptor (P4-R), and the β2 receptor (β2-R), thereby regulating luteal steroidogenesis. Two populations of luteal cells were used in the present study: from 6th–10th and 11th–16th days of the estrous cycle, which were isolated from corpus luteum (CL) from slaughtered cows. The luteal cells were treated for six hours with one of the following hormones: luteinizing hormone (LH; 100 ng/ml); progesterone (P4; 10 –5M); progesterone antagonist (aP4; 10 –5M); noradrenaline (NA; 10–5M); or actinomycin D (ActD; 500 ng/ml). After treatment, the medium was collected for the determination of progesterone concentration. With LH, the P4 concentration in the medium increased with both 6 th–10th and 11th–16th days. None of the other treatments affected the progesterone concentration of the medium. The level of expression of the genes for OT-R, P4-R and β2-R were determined. Total RNA was extracted from cells, treated with DNase, and subjected to reverse transcription. Treatment with luteinizing hormone was the only treatment that increased the level of expression of the gene for P4-R in both 6 th–10th and 11th–16th days of the estrous cycle. Both treatment with luteinizing hormone and treatment with progesterone increased the level of expression of the gene for OT-R in 6th–10th days. The basal level of expression of the gene for OT-R was higher in 6th–10th days than in 11th–16th days. This suggests that there is positive feedback between progesterone and oxytocin, with both playing a role as a local, intra-ovarian factor that enhances the function of the corpus luteum.