Equine arteritis virus-induced polypeptide synthesis.

Geographic Distribution of Equine Arteritis Virus Clinical Outcome of Equine Arteritis Virus Infection How Equine

Equine Viral Arteritis

Equine viral arteritis (EVA) is a contagious viral disease of equids caused by equine arteritis virus (EAV), an RNA virus that is classified in the family Arteriviridae. The virus is found in horse populations in many countries worldwide. EVA cannot be differentiated clinically from a number of other respiratory and systemic equine diseases. While laboratory-confirmed outbreaks of EVA have been...

Characterization of an equine arteritis virus replicase mutant defective in subgenomic mRNA synthesis.

Equine arteritis virus (EAV) is a positive-stranded RNA virus that synthesizes a 5'- and 3'-coterminal nested set of six subgenomic mRNAs. These mRNAs all contain a common leader sequence which is derived from the 5' end of the genome. Subgenomic mRNA transcription and genome replication are directed by the viral replicase, which is expressed in the form of two polyproteins and subsequently pro...

Equine arteritis virus-neutralizing antibody in the horse is induced by a determinant on the large envelope glycoprotein GL.

Complementary DNAs encoding ORFs 2 to 7 equine arteritis virus (EAV) have been cloned into the expression vector pGEX to produce glutathione-S-transferase fusion proteins. Recombinant proteins were affinity purified and screened in ELISA with equine sera to identify immunoreactive polypeptides. The large envelope glycoprotein (GL) was identified as the most reactive to EAV-positive equine sera ...

Importance of M-protein C terminus as substrate antigen for serodetection of equine arteritis virus infection.

Equine arteritis virus (EAV), an enveloped positive-stranded RNA virus, is the prototype of the arterivirus group. In a previous paper (A. Kheyar, S. Martin, G. St.-Laurent, P. J. Timoney, W. H. McCollum, and D. Archambault, Clin. Diagn. Lab. Immunol. 4:648-652, 1997), we have shown that the unglycosylated membrane (M) protein, which is composed of 162 amino acids (aa), is a major target of equ...