Purification and iodination of antibody for use in an immunoradiometric assay for serum ferritin.
نویسنده
چکیده
Antibodies were purified by affinity chromatography and radioiodinated by a conjugation method for use inthe immunoradiometric assay for serum ferritin. These procedures are simpler and more reproducible than those described earlier, and the radiolabeled preparations were usable for at least 13 weeks.
منابع مشابه
A novel immunoradiometric assay for human liver ferritin.
Rivanol, the cationic salt of an acridine base, has been used as a novel separation procedure in an immunoradiometric assay for human liver ferritin. The separation step is based on the differences in charge and molecular weight between the labelled antibody-ferritin complex and free labelled immunoglobulins. The resultant assay is simple, reproducible and sufficiently sensitive to determine se...
متن کاملPreparation of an artificial matrix to replace human standards of prostate specific antigen IRMA assay kit [Persian]
Introduction: Prostate specific antigen (PSA) is one of the most sensitive markers for diagnosis of prostate cancer. Immunoradiometric kit (IRMA) is a common and sensitive method for determination of PSA in clinical laboratories. This kit has four major components: solid phase coated with monocolonal antibody, pair antibody labelled with I-125, series of standards i...
متن کاملPreparation of Human Liver Ferritin
Ferritin is the major intracellular iron storage protein found in the body, consisting of a spherical protein shell of MW 450 000 made up of 24 identical subunits. Iron is stored in the inner core as crystalline ferric-oxide-phosphate. Ferritin is found mainly in the reticuloendothelial system, predominantly in the spleen, liver, and bone marrow. Circulating levels of ferritin have been shown t...
متن کاملPreparation of prostate specific antigen standards for immunoradiometric assay
Background: Immunoradiometric assay is one of the most common and precise methods for determination of prostate specific antigen (PSA) in clinical laboratories. Usual use of human serum in routine assays has many disadvantages such as easy contamination and precipitation, instability and unavailability. Thus in order to avoid these problems the artificial matrix was used which acts sim...
متن کاملAn immunoradiometric assay for ferritin in the serum of normal subjects and patients with iron deficiency and iron overload.
An immunoradiometric assay for human ferritin has been developed. Concentrations of ferritin in the serum of male and female controls and patients with iron deficiency and iron overload were measured.Male controls were found to have a significantly higher mean concentration of serum ferritin than females. Patients with iron deficiency had significantly lower levels than normals of either sex an...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 23 2 PT. 1 شماره
صفحات -
تاریخ انتشار 1977