The Microdetermination of Biological Copper with Oxalyldihydrazide.

نویسندگان

  • R N BEALE
  • D CROFT
چکیده

Methods are described for the determination of microgram quantities of biological copper in aqueous medium as the intensely coloured oxalyldihydrazide-acetaldehyde complex (molar extinction coefficient 23,000 to 23,500). The methods are applicable on a routine basis to any biological material, in particular to serum, urine, or tissues, such as liver, brain, or kidney. In the case of urine a simple semi-quantitative screening method is also described. For quantitative work copper is liberated from serum by acid extraction and protein precipitation, and from urine and tissues by a rapid wet-ashing procedure. Recoveries of added copper from urine are quantitative. Precision is high although day-to-day control with standards and blanks is desirable. The methods are applied in an investigation of the diurnal variations in a normal man and in two cases of Wilson's disease.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Ziziphus mauritiana mediated synthesis of copper and nickel nanoparticles for comparative efficacy in biological water purification

The burden of life on the earth is the source of biological contamination in water. Nanotechnology has promising contributions in control of microbial contaminations and medicinal plants further increase these properties. Presently, copper acetate and nickel oxide nanoparticles were synthesized using 1mM solution of each with Ziziphus mauritiana leaves extract as reducing agent. Nanoparticles w...

متن کامل

Colorimetric Microdetermination of Free Fatty Acids1

A colorimetric method for determination of free fatty acids has been developed, based on the estimation of copper in a chloroform extract of their cupric salts with oxalic acid bis-(cyclohexylidenehydrazide). Contamination with inorganic copper during extraction is practically eliminated by using filter-paper pads. Specificity and interferences are discussed. Recoveries average 101.1%. Normal v...

متن کامل

Hybrid Nanocomposites of Montmorillonite/Copper Oxide, Synthesis and Evaluation as Effective Growth Inhibitors in Different Biological Systems

The external and interlamellar spaces of montmorilonite (MMT) were used as solid support for synthesis of CuO nanoparticles (NPs) at room temperature by the chemical reduction method. In this project, Copper Nitrate plus water (Cu(NO3)2. xH2O) and Sodium Hydroxide (NaOH) were used as Copper precursor and reducing agent respectively. Then, MMT/Cu2+ nanocomposites were stabilized with different r...

متن کامل

Hybrid Nanocomposites of Montmorillonite/Copper Oxide, Synthesis and Evaluation as Effective Growth Inhibitors in Different Biological Systems

The external and interlamellar spaces of montmorilonite (MMT) were used as solid support for synthesis of CuO nanoparticles (NPs) at room temperature by the chemical reduction method. In this project, Copper Nitrate plus water (Cu(NO3)2. xH2O) and Sodium Hydroxide (NaOH) were used as Copper precursor and reducing agent respectively. Then, MMT/Cu2+ nanocomposites were stabilized with different r...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of clinical pathology

دوره 17  شماره 

صفحات  -

تاریخ انتشار 1964