Cryopreservation of Embryos of the New World Screwworm <i>Cochliomyia hominivorax</i> (Diptera: Calliphoridae)
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چکیده
A method for cryopreserving embryos of the screwworm, Cochliomyia hominivorax (Coquerel), was developed for the long-term storage of strains used in research projects and for colonies maintained as back-up to production strains that are mass-reared for sterile insect release programs. This protocol, when combined with a previous procedure that was designed to render screwworm embryos permeable to water loss and the inßux of cryoprotectants, yields rates of '53% hatching, 22% pupation, and 75% adult emergence. These yields are all lower than control levels, but by the next generation the progeny of the cryopreserved ßies are near or equal to control levels and are available in sufÞcient numbers to reestablish laboratory colonies. Important to the success of this studywas identifying theoptimumembryonic stage for treatment, formulating an incubation regime, which allowed consistent retrieval of embryos at the optimum stage, establishing the correct dehydration time for the previtriÞcation step, and developing a recovery systemafter liquid nitrogen storage of the embryos.
منابع مشابه
Permeabilization of Cochliomyia hominivorax (Diptera: Calliphoridae) embryos.
Embryos of the primary screwworm, Cochliomyia hominivorax (Coquerel), were successfully permeabilized for use in subsequent cryopreservation studies. Mortality was greater for eggs incubated for < 5 h before treatment. The mean survival of embryos to first instars was 55.7, 61.1, and 62.6% when the embryos were incubated for 5, 5.5, and 6 h before treatment, respectively. The survival to the pu...
متن کاملPHYSIOLOGY, BIOCHEMISTRY, AND TOXICOLOGY Cryopreservation of Embryos of the New World Screwworm Cochliomyia hominivorax (Diptera: Calliphoridae)
A method for cryopreserving embryos of the screwworm, Cochliomyia hominivorax (Coquerel), was developed for the long-term storage of strains used in research projects and for colonies maintained as back-up to production strains that are mass-reared for sterile insect release programs. This protocol, when combined with a previous procedure that was designed to render screwworm embryos permeable ...
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The primary screwworm fly Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) is one of the most important insect pests of livestock in neotropical regions, whereas Cochliomyia macellaria (Fabricius) (Diptera: Calliphoridae), the secondary screwworm, is of medical and sanitary importance because of its role in the dissemination of pathogens. These two species share morphological similar...
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Sterile insect technique (SIT) programs are designed to eradicate pest species by releasing mass-reared, sterile insects into an infested area. The first major implementation of SIT was the New World Screwworm Eradication Program, which successfully eliminated the New World screwworm (NWS), Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae), from the Continental US, Mexico and much of ...
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