Real - time TaqMan PCR for Yersinia enterocolitica detection based on 1 the ail and foxA genes
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چکیده
1 Corresponding author. Mailing address: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, 155 Changbai Road Changping District 102206, Beiing, People’s Republic of China. Phone: 8610 58900758. Fax: 8610-58900070. E-mail: [email protected], [email protected] a J. W., R. D., J. L., and Y. H., Contributed equally to this study. JCM Accepts, published online ahead of print on 22 October 2014 J. Clin. Microbiol. doi:10.1128/JCM.02528-14 Copyright © 2014, American Society for Microbiology. All Rights Reserved.
منابع مشابه
Real-time TaqMan PCR for Yersinia enterocolitica detection based on the ail and foxA genes.
Yersinia enterocolitica, a cause of emerging enteric infections, is a foodborne pathogen associated with various enteric and systemic syndromes, e.g., diarrhea, enteritis, and enterocolitis. Therefore, the detection of this pathogen has important significance. Previous real-time PCR for detection of Y. enterocolitica was primarily based on the ail gene; biotype 1A nonpathogenic strains were not...
متن کاملComparison of culture, multiplex, and 5' nuclease polymerase chain reaction assays for the rapid detection of Yersinia enterocolitica in swine and pork products.
Bacteriological culture was compared with multiplex and fluorogenic (TaqMan) polymerase chain reaction (PCR) assays for the detection of attachment invasion locus (ail)-bearing Yersinia enterocolitica in market weight swine, chitterlings, and ground pork. The TaqMan assay detected 1 pg of purified Y. enterocolitica DNA, whereas conventional gel-based PCR detected I ng of the same. The presence ...
متن کاملThe detection of Yersinia enterocolitica in surface water by quantitative PCR amplification of the ail and yadA genes.
Yersinia enterocolitica has been detected in surface water, and drinking untreated water is a risk factor for infection. PCR-based methods have been used to detect Y. enterocolitica in various sample types, but quantitative studies have not been conducted in water. In this study, quantitative PCR (qPCR)-based methods targeting the Yersinia virulence genes ail and yadA were used to survey the Gr...
متن کاملبررسی میزان شیوع آئروموناس هیدروفیلا و یرسینیا انتروکولیتیکا در کودکان مبتلا به اسهال حاد مراجعه کننده به مراکز بهداشتی و درمانی شهرستان همدان
Introduction & Objective: Diarrhea is the most common cause of morbidity and mortality in all age groups, especially children, the elderly and immunocompromised patients. Various studies have been reported regarding the relationship between the children acute diarrhea and Aeromonashydrophila and Yersinia enterocolitica. This study aimed to investigate the prevalence of the bacteria and their se...
متن کاملDevelopment of a PCR-based method for detection of pathogenic Yersinia enterocolitica in pork
Thisted Lambertz, S. 2005. Development of a PCR-based method for detection of pathogenic Yersinia enterocolitica in pork. Doctor ́s dissertation. ISSN 1652-6880, ISBN 91-576-6922-8 During the last decade, Yersinia enterocolitica has been reported to cause between 500 and 800 cases of human gastroenteritis per year in Sweden. As pigs are the only animals in human food production that regularly ha...
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تاریخ انتشار 2014