Extraction of Microsomal Fraction from Midguts of Western Spruce Budworm Choristoneura occidentalTM F., and Rat Liver, and their Ability to Expoxidize Aldrin
نویسنده
چکیده
Microsomal fractions were isolated from midguts of western spruce budworm larvae and rat liver. The microsomal preparations were then assayed for cytochrome P-450 content and aldrin epoxidase activity. Both microsomal preparations showed distinct spectrum of cytochrome P-450. Relative to protein content, the cytochrome P-450 content of midguts of western spruce budworm was lower by 7 — 9 times compared to that of the rat liver. Epoxidase activity is present in the two microsomal preparations as indicated by their ability to convert aldrin to dieldrin. The activity of both microsomal preparations was inhibited by piperonyl butoxide.
منابع مشابه
Composition of the Spruce Budworm (Choristoneura fumiferana) Midgut Microbiota as Affected by Rearing Conditions
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