PASADENA hyperpolarization: Instrumentation and preparation of tracers for in vivo application

Motivation In the dawn of hyperpolarization (HP) of molecules in solution for biomedical application, the question of suitable instrumentation is imperative. Dynamic Nuclear Polarization (DNP) polarizers are commercially available, but rather cost-intensive. Nevertheless, DNP is used by an increasing number of laboratories, while Parahydrogen And Synthesis Allow Dramatically Enhanced Nuclear Alignment (PASADENA) [1, 2] is presently installed only in very few places (<5). This may be attributed to the fact that only few molecules and no instrumentation for PASADENA of biomolecules are commercially available or published in scientific literature. Here, we present a polarizer for the reliable hyperpolarization of a variety of molecules, including novel biomolecule 1-C, 2,3-D2 Succinate [7] and other molecular agents.