Eletrochemical immunosensor development for detection of dengue
نویسندگان
چکیده
Background The development of biosensors for the diagnosis and monitoring of diseases, drug discovery, proteomics, and the environmental detection of pollutants and/or biological agents is an extremely significant problem. Fundamentally, a biosensor is derived from the coupling of a ligand-receptor binding reaction to a signal transducer. In this study we used as a model a peptide marker of DENV (dengue virus) type 1. The electrochemical technique of cyclic voltammetry was performed to detect the signal generated by the interaction between the peptide and antibody from patients’ blood samples. Graphite and gold electrodes modified with chitosan film were employed to evaluate the interaction antigen-antibodies. The construction of this immunosensor, able to identify in real time circulating antibodies or antigens can be applied in the diagnosis of dengue and other infectious and parasitic diseases.
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