Inhibition of IgE and IgE/anti-IgE mediated responses in mast cells by Omalizumab
نویسندگان
چکیده
Results Omalizumab (10 ug/mL) inhibited IgE binding to LAD2 cells by 78% (P=0.007) compared to untreated control. Omalizumab (10 ug/mL) further blocked IgE-dependent upregulation of FcεRI expression by 90% (P=0.03). In addition, omalizumab removed FcεRI-bound IgE in a time-dependent manner; an effect that was detected as early as 24 hrs (57% removal; P<0.001) after addition of omalizumab resulting in a concomitant decrease in IgEdependent FcεRI expression (30%; P<0.001). Omalizumab attenuated degranulation induced by anti-IgE crosslinking of bound IgE in a dose dependent manner, with 66% inhibition (P<0.0001) at 25 ug/ml. Furthermore, 100 ug/ml omaluzimab prevented cysteinyl leukotriene production and FcεRI–dependent modulation of TLR expression.
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