Validation of in vivo translocation frequency obtained by FISH with conventional G-banding Evaluation of ionizing radiation exposure for biodosimetry: Validation of Whole Chromosome

Construction of dicentric and translocation dose response curves by the FISH assay. Evaluation of ionizing radiation exposure for biodosimetry: Validation of Whole Chromosome

Application of conventional chromosomal aberration and fluorescence in situ hybridisation (FISH) translocation in the assessment of occupationally derived irradiation

Background: Most of our current understanding of the biological effects of exposure to ionising radiation is based on conventional cytogenetic techniques, which enable us to determine the relationship between chromosomal aberration and dose received by radiation workers. However, conventional techniques have numerous limitations and chromosomal aberrations can be easily missed. Since FISH plays...

Prompt cytomolecular identification of chromosome aberration in irradiated blood cells

Background: understanding the genomic alteration induced by ionizing radiation still remains to be a methodological challenge in genetic field. The energy released from this type of radiation can potentially causes structural and numerical alterations in lymphocytes, which in turn converts them into abnormal tumor cells. Chromosomal abnormalities associated with specific type of hematological m...

Strong Correlation among Three Biodosimetry Techniques Following Exposures to Ionizing Radiation

Three in vitro dose calibration curves for biodosimetry such as dicentric chromosome assay, fluorescence in situ hybridization (FISH) assay for translocation, and micronuclei (MNs) in binucleated cell assay were established after exposure to ionizing radiation. Peripheral blood lymphocyte samples obtained from healthy donors were irradiated with 60Co source at a dose rate of 0.5 Gy/min to doses...

Evaluation of Chromosomal Aberrations Among Radiotherapy Staff in the City of Tehran Because of Occupational Exposure Using Micronucleus Assay and GTG-Banding

Introduction: Exposure to ionizing radiation as a result of diagnostic, treatment and occupational reasons, has created a remarkable range of cytogenetic damage in the DNA, as the first and main goal of ionizing radiation. The aim of this study is to assess the chromosomal damage in medical radiotherapy workers and its association with effective parameters such as age, sex, duration of employme...