Effect of phytohemagglutinin in cultures on the lactate dehydrogenases of lymphocytes from chronic lymphatic leukemia.
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چکیده
By YALE RABINOWITL AND ALBERT A. D rz With the technical assistance of Tina Lubrano, Inge Schimo and Betty A. Wilhite II ESULTS OF A STUDY OF CHANGES in lactic dehydrogenase (LDH) occurring in cultures of normal human lymphocytes were reported recently.’ It was shown that lymphocytes in cultures without phytohemagglutinin (PHA) showed little change in total LDH activity or in isozyme patterns; but when cultured with PHA, significant increases resulted in the total LDH activity and in the proportion of muscle-type (M-LDH) polypeptide subunits. These changes, as well as blast-cell formation, were blocked by either actinomycin D or puromycin. This indicated that both RNA and protein synthesis were required for the PHA effect. Changes in LDH isozyme patterns were detectable after four hours of culture and became highly significant within 24 hours. Since increased mitotic activity usually does not begin until the second to third day of culture with PHA, the changes in LDH occurred in the same cells that were introduced into the cultures and were not the result of the appearance of a new population of cells. As highly purified lymphocyte preparations were used in these cultures, the results were not due to a change in the ratio of lymphocytes to granulocytes. This is important since granulocytes have a much greater total LDH activity and higher proportion of M-LDH than do lymphocytes.2 6 These results indicated that PHA acted at the two genetic loci controlling synthesis of M-LDH and H-LDH (heart-type) polypeptide subunits. It was suggested that perhaps PHA acted at allosteric sites on protein repressors or inducers of operator genes for Hand M-LDH in accordance with the theories of Monod et al.7 In the present study, we are reporting observations of changes in LDH in
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ورودعنوان ژورنال:
- Blood
دوره 31 2 شماره
صفحات -
تاریخ انتشار 1968