RapiGest SF Surfactant: An Enabling Tool for In-Solution Enzymatic Protein Digestions

The mechanism by which RapiGest SF improves the speed and completeness of digestion is illustrated in Figure 1. Mild protein denaturation opens protein structure and exposes the proteolytic sites to enzymatic cleavage. Enzymes are more resistant to denaturation than common proteins and remain active in RapiGest SF solutions. More complete denaturation of globular proteins can be accomplished by heating in a RapiGest SF solution at elevated temperatures prior to the addition of enzymes and incubating the sample with the enzyme at 37 °C.1,2