Factors Affecting Plaque Formation by the Infectious Ribonucleic Acid of the Equine Encephalitis Viruses.

Effects of poly-L-lysine on infectious viral nucleic acid.

Infectious ribonucleic acids (IRNA) of Venezuelan equine encephalitis and Eastern equine encephalitis viruses were observed to form noninfectious complexes with a basic polyamino acid, poly-l-lysine. Original infectivity was recovered from the complexes by digestion of the polylysine with Pronase, and partial recovery was effected by treatment with sodium dodecyl sulfate. Infectivity could not ...

The Formation of Plaques by African Horse Sickness Viruses and Factors Affecting Plaque Size

Comparison of starch, agarose, and agar for plaquing Venezuelan encephalitis virus.

Although agar has been widely used for plaque assays of animal viruses, it is an ill-defined substance containing acid polysaccharides that inhibit growth of some viruses, including arboviruses (H. Liebhaber and K. K. Takemoto, Virology 14:502, 1961; I. T. Schulse and R. W. Schlesinger, Virology 19:49, 1963). Thus, it was necessary for studies of Venezuelan encephalitis (VE) virus in chicken em...

Temperature-sensitive steps in the biosynthesis of Venezuelan equine encephalitis virus.

In contrast to Eastern equine encephalitis virus, the replication of Venezuelan equine encephalitis (VEE) virus was strongly inhibited at 44 C in chick embryo cells. The inhibited steps were analyzed by shifting the incubating temperatures up or down, and by determining during the shifts the rate and extent of infectious ribonucleic acid (RNA) synthesis, intact virus synthesis, and formation of...

Altered viral ribonucleic acid synthesis by a temperature-sensitive mutant of Eastern equine encephalitis virus.