Combining Electrowetting and Magnetic Bead Manipulations for Use in Microfluidic Immunoassays
نویسندگان
چکیده
This experiment investigated reducing the cost of microfluidic immunoassays. Magnetic microbeads are used in these assays to increase the efficiency of immunoassays, but they also raise the cost. This experiment aims to reduce the number of magnetic beads necessary by using electrowetting in conjunction with the beads. Single and parallel plate electrowetting devices were tested individually and the magnetic microbeads. The devices did not succeed in separating the beads from the droplets, but the results show many promising options for further investigation.
منابع مشابه
Heterogeneous immunoassays using magnetic beads on a digital microfluidic platform.
A digital microfluidic platform for performing heterogeneous sandwich immunoassays based on efficient handling of magnetic beads is presented in this paper. This approach is based on manipulation of discrete droplets of samples and reagents using electrowetting without the need for channels where the droplets are free to move laterally. Droplet-based manipulation of magnetic beads therefore doe...
متن کاملHigh-throughput Patterning of Single Magnetic Beads Using Digital Microfluidic Technology
We present a novel strategy for patterning single magnetic beads by using digital microfluidics (DMF). A droplet containing a suspension of superparamagnetic particles is transported back and forth over an array of femtoliter-sized microwells by using electrowetting-on-dielectric actuation forces. Compared to existing methods, this technique allows patterning of superparamagnetic beads in micro...
متن کاملAutomated electrotransformation of Escherichia coli on a digital microfluidic platform using bioactivated magnetic beads.
This paper reports on the use of a digital microfluidic platform to perform multiplex automated genetic engineering (MAGE) cycles on droplets containing Escherichia coli cells. Bioactivated magnetic beads were employed for cell binding, washing, and media exchange in the preparation of electrocompetent cells in the electrowetting-on-dieletric (EWoD) platform. On-cartridge electroporation was us...
متن کاملPotential Use of DNA Aptamer-Magnetic Bead Separation-PCR Assay for Salmonella Detection in Food
Background: Salmonella is one of the most common food-borne pathogens that can cause illness. In this study, the sensitivity and the specificity of Aptamer-Magnetic bead Separation-Polymerase Chain Reaction (AMS-PCR) method were determined for Salmonella spp. detection. Methods: Different concentrations of Salmonella enterica were mixed with streptavidin-magnetic beads coated with biotinylated...
متن کاملFerromagnetic Particles for an Improved Heterogeneous Bioassay Performance on a Digital Lab-on-a-chip
In this work, we report on a novel strategy to implement and to optimize heterogeneous bioassays on an electrowetting-on-dielectric (EWOD) digital lab-on-a-chip analysis platform, namely the use of ferromagnetic particles as functional carriers for biomolecules in combination with a highly efficient extraction protocol. This extraction protocol serves as an extension to the common operations of...
متن کامل