Measurement of the cyanophycin granule polypeptide contained in the blue-green alga Anabaena cylindrica.
نویسنده
چکیده
An assay was developed to determine the amount of cyanophycin granules in blue-green algae. The amount of this polypeptide in cells of Anabaena cylindrica was measured as a function of culture age and was compared with changes in other proteinaceous cellular components. The data presented support the notion that the cyanophycin granule is a cellular nitrogen reserve.
منابع مشابه
Cyanophycin Granules from the Blue-Green Alga Anabaena cylindrica: A Reserve Material Consisting of Copolymers of Aspartic Acid and Arginine.
The cyanophycin granules from two-week-old cultures of the blue-green alga Anabaena cylindrica were isolated by sonication of the cells followed by differential centrifugation. These particles are composed of polypeptides containing only two amino acids, aspartic acid and arginine, and have a molecular weight between about 25,000 and 100,000.
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Methods have been developed for identifying the pathway of assimilation of N2-derived nitrogen. The products of fixation of 13N-labeled nitrogen gas ([13N]N2), and the distribution of 13N within glutamine, were determined after short periods of labeling (approximately 1 to 120 s) and also in pulse-chase experiments. Ammonia, the amide nitrogen of glutamine, and the alpha-amino nitrogen of gluta...
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Arginine decarboxylase produces agmatine, and arginase and agmatinase are ureohydrolases that catalyze the production of ornithine and putrescine from arginine and agmatine, respectively, releasing urea. In the genome of the filamentous, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, ORF alr2310 putatively encodes an ureohydrolase. Cells of Anabaena supplemented with [(14) C]ar...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 114 3 شماره
صفحات -
تاریخ انتشار 1973