The Supravital Staining of Vaccine Bodies

Revision of the diagnosis of a case of hereditary hemolytic anemia by supravital staining.

A 20-year-old splenectomizedwomanwith hemolytic anemia, diagnosed at the age of 3 years with congenital dyserythropoietic anemia type I, was referred to our institution for re-examination of 2 unusual features: high red blood cell (RBC) pyruvate kinase (PK) activity and methemoglobinemia. On examination, she showed 3.5% methemoglobin and unusually high PK activity (45.0 U/gHb). Other RBC enzyme...

On-spot brilliant cresyl blue staining in fine needle aspiration, a novel technique for cytological diagnosis.

OBJECTIVE Application of on-spot staining with brilliant cresyl blue, a supravital stain, was investigated in fine needle aspirates from breast, lymph node and thyroid. STUDY DESIGN Patients with palpable breast lumps, lymph nodes and thyroid nodules were included in the study. Brilliant cresyl blue was used to stain the smears, which were evaluated for smear adequacy as well as cytological d...

P-6: Flow Cytometry: Assessment of Protamine Deficiency by CMA3 Staining and Apoptotic Bodies

Background: Chromomycin A3 (CMA3) staining, with the slide method or fluorescence microscopy, is widely used for indirect assessment of protamine deficiency in a semen sample. Flow cytometry is the most suitable tool which to improve assessment accuracy, both in terms of statistical analysis and to prevent observer variation. This study provides a simple procedure to account for merocyanine 540...

Determination of membrane integrity of canine spermatozoa

Semen samples from 9 sexually mature dogs of different breeds, aged from 1 to 5 years were used. Quality of sperm was evaluated using the following parameters: progressive motility (subjective), then structural and functional integrity of spermal membrane (supravital colouring (eosine/nigrosine) and HOS test). The majority of HOS reactive or swollen spermatozoa was placed in a hypoosmolar solut...

Flow Cytometry: A Novel Approach for Indirect Assessment of Protamine Deficiency by CMA3 Staining, Taking into Account the Presence of M540 or Apoptotic Bodies