MicroRNA precursors in motion: exportin-5 mediates their nuclear export.
نویسنده
چکیده
The discovery of microRNSs (miRNAs) has changed the paradigm of gene regulation, leaving us with numerous exciting questions regarding what these molecules do and how they originate. A model for miRNA biogenesis has emerged recently, yet several key factors--including the identity of the miRNA nuclear export receptor--remained unknown. However, recent studies have shown that exportin-5 (Exp5), a Ran-dependent importin-beta-related transport receptor, mediates nuclear export of miRNA precursors (pre-miRNAs).
منابع مشابه
Structural requirements for pre-microRNA binding and nuclear export by Exportin 5.
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MicroRNAs (miRNAs), which function as regulators of gene expression in eukaryotes, are processed from larger transcripts by sequential action of nuclear and cytoplasmic ribonuclease III-like endonucleases. We show that Exportin-5 (Exp5) mediates efficient nuclear export of short miRNA precursors (pre-miRNAs) and that its depletion by RNA interference results in reduced miRNA levels. Exp5 binds ...
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Abstract The biogenesis of most eukaryotic kinds of RNA requires nuclear export, which is mediated by a variety of specific nuclear transport receptors. The nuclear export receptors Exportin-t (Exp-t) and Exportin 5 (Exp5), and their homologues, are involved in the export of transfer RNA to the cytoplasm. Exp5 is further involved in additional nucleocytoplasmic transport pathways, which include...
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MicroRNAs (miRNAs) are initially expressed as long transcripts that are processed in the nucleus to yield approximately 65-nucleotide (nt) RNA hairpin intermediates, termed pre-miRNAs, that are exported to the cytoplasm for additional processing to yield mature, approximately 22-nt miRNAs. Here, we demonstrate that human pre-miRNA nuclear export, and miRNA function, are dependent on Exportin-5....
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ورودعنوان ژورنال:
- Trends in cell biology
دوره 14 4 شماره
صفحات -
تاریخ انتشار 2004