Calpain-10 Gene Polymorphism in Type 2 Diabetes Mellitus Patients in the Gaza Strip

Objective: To examine the role of calpain-10 SNP-44, -43, -63 and del/ins-19 in genetic susceptibility to type 2 diabetes mellitus (T2DM) and associations with triglycerides and total cholesterol in a group of subjects residing in the Gaza Strip. Subjects and Methods: Ninety-six individuals were examined: 48 T2DM patients and 48 controls. The groups were genotyped for calpain-10 SNP-44, -43, -63, and del/ins-19. Mutagenically separated polymerase chain reaction was used to examine SNP-44; del/ins-19 was examined by electrophoresis of the PCR product on agarose gel, while the restriction fragment length polymorphism method was used for SNP-43 and -63. Results: There was evidence that the C allele at SNP-44 played a possible role in susceptibility to T2DM (p = 0.01). T2DM patients with G/A genotype were found to have higher levels of total cholesterol in comparison to those homozygous for allele 1 (G/G) in SNP-43. Total cholesterol levels increased in T2DM patients who are homozygous for del/ins-19 allele 2, in T2DM patients with the 121/221 haplotype combination, and in control subjects with the haplotype combination 111/121. Conclusion: SNP-44 Received: February 15, 2009 Revised: August 13, 2009 Mr. Mazen M. Zaharna Department of Medical Technology Islamic University of Gaza PO Box 108, Gaza City (Palestine) Tel. +970 59 960 4870, Fax +970 8 286 0800, E-Mail mzaharna @ iugaza.edu.ps © 2010 S. Karger AG, Basel 1011–7571/10/0196–0457$26.00/0 Accessible online at: www.karger.com/mpp D ow nl oa de d by : 54 .7 0. 40 .1 1 11 /1 9/ 20 17 1 2: 28 :0 6 P M