Multi-enzyme complexes on DNA scaffolds capable of substrate channelling with an artificial swinging arm.

Multi-enzyme complexes on DNA scaffolds capable of substrate channeling with an artificial swinging arm

Metabolite Channelling and Metabolic Complexity Substrate channelling in 2-0x0 acid dehydrogenase multienzyme complexes

Heteronuclear NMR spectroscopy and other experiments indicate that the true substrate of the E l component of 2-0x0 acid dehydrogenase complexes is not lipoic acid but the lipoyl domain of the E2 component. E l can recognize the lipoyllysine residue as such, but reductive acylation ensues only if the domain to which the lipoyl group is attached is additionally recognized by virtue of a mosaic o...

Swinging arms in multifunctional enzymes and the specificity of post-translational modification.

Introduction Covalently attached prosthetic groups serve as swinging arms in several different and wellcharacterized multifunctional enzymes. Notable among these are the lipoyl-lysine residues in 2-0x0 acid dehydrogenase multienzyme complexes [l-31 and the glycine cleavage system [4-61, the biotinyl-lysine residues in various ATP-dependent carboxylases [7,8] and the phosphopantetheinyl-serine r...

Molecular architecture of the pyruvate dehydrogenase complex: bridging the gap.

The PDC (pyruvate dehydrogenase complex) is a high-molecular-mass (4-11 MDa) complex of critical importance for glucose homoeostasis in mammals. Its multi-enzyme structure allows for substrate channelling and active-site coupling: sequential catalytic reactions proceed through the rapid transfer of intermediates between individual components and without diffusion into the bulk medium due to its...

Fine-tuning of microsolvation and hydrogen bond interaction regulates substrate channelling in the course of flavonoid biosynthesis.

In the course of metabolite formation, some multienzymatic edifices, the so-called metabolon, are formed and lead to a more efficient production of these natural compounds. One of the major features of these enzyme complexes is the facilitation of direct transfer of the metabolite between enzyme active sites by substrate channelling. Biophysical insights into substrate channelling remain scarce...