Comparison of proteins expressed on secretory vesicle membranes and plasma membranes of human neutrophils.

Membranes of Human Neutrophils Secretory Vesicle Membranes and Plasma Comparison of Proteins Expressed On

The lysosomal membrane glycoproteins Lamp-1 and Lamp-2 are present in mobilizable organelles, but are absent from the azurophil granules of human neutrophils.

The subcellular localization of two members of a highly glycosylated protein group present in lysosomal membranes in most cells, the lysosome-associated membrane proteins 1 and 2 (Lamp-1 and Lamp-2), was examined in human neutrophil granulocytes. Antibodies that were raised against purified Lamp-1 adn Lamp-2 gave a distinct granular staining of the cytoplasm upon immunostaining of neutrophils. ...

Human neutrophils are devoid of the integral membrane protein caveolin.

The secretory vesicles of human neutrophils are rapidly mobilizable vesicles that contain several GPI-linked proteins, a characteristic feature of caveolae in other cells. To investigate whether secretory vesicles are structurally related to caveolae, we examined human neutrophils for the presence of caveolin, a major constituent of caveolae, by immunoblotting using monoclonal and polyclonal an...

Comparison of Biocompatibility of Various Membranes with Fibroblasts

  Objective: Different techniques have been suggested for the repair of bone defects at the injured sites. Use of biomembranes, or application of plasma rich in growth factor (PRGF) at the site of proliferation of osteoblasts are among the suggested techniques. The current study aimed to compare the biocompatibility of human periodontal ligament fibroblasts (hPLF) cultured on Hypro-Sorb F, Peri...

Neutrophil secretory vesicles are the intracellular reservoir for GPI-80, a protein with adhesion-regulating potential.

The subcellular localization of GPI-80, a novel, adhesion-regulating protein, was investigated in human neutrophils. Surface expression of GPI-80 was determined by FACS analysis as well as by the ability for phospholipase C to cleave the protein from the cell surface. Increasing amounts of GPI-80 were exposed on the cell surface after weak stimulation with the chemoattractant fMLF, suggesting t...