serological and molecular diagnosis of some viral diseases infecting chickpea (cicer arietinum) in lorestan and hamadan provinces in iran

legume cultivation and production including chickpea is one of the main agricultural objectives in iran. chickpea crop is a good sources of protein consumption for human diet and play a significant role in nitrogen fixation in cultivated soil and also self reliance for iran economy. chickpea cultivated as a non irrigated crop in lorestan and hamadan provinces in iran. several virus diseases infecting chickpea  and causing loss to the crop at growing stages in the field. during the year 2014 , several chickpea samples before the flowering and pod set stages with symptoms of general yellowing often with stunting, dwarfing, bronzing of leaf margin, narrow leaf, browning of  inner stem ,were collected. serological tests including elisa and tissue blot (tiba) indicated that some samples were infected by luteoviruses of cpcsv, blrv, sbdv, bwyv and the nanovirus fbnyvv. to confirm the serological results, rt-pcr  test were carried using the general set of primers for luteovirus amplification. viral cdna were amplified in thermocycler which resulted in a specific band of about 530bp in agarose gel.