When High Throughput Meets Mechanistic Studies

When High Throughput Meets Mechanistic Studies

The greatest insights in science are obtained through integrative approaches that combine the best aspects emerging and traditional technologies. The study by Veerman et al that appears in this issue of Circulation Research thoughtfully integrates big data high throughput technology, small data molecular approaches, and electrophysiological methods to draw conclusions important both to the unde...

Glycomics meets genomics, epigenomics and other high throughput omics for system biology studies.

Majority of eukaryotic proteins are glycosylated and their glycan moieties have numerous important structural, functional and regulatory roles. Because of structural complexity of glycans and technological limitations glycomics, and particularly glycoproteomics was not able to follow rapid progress in genomics and proteomics over last 30 years. However, the field of glycan has been progressing ...

Development of a universal radioactive DNA methyltransferase inhibition test for high-throughput screening and mechanistic studies

DNA methylation is an important epigenetic mark in eukaryotes, and aberrant pattern of this modification is involved in numerous diseases such as cancers. Interestingly, DNA methylation is reversible and thus is considered a promising therapeutic target. Therefore, there is a need for identifying new small inhibitors of C5 DNA methyltransferases (DNMTs). Despite the development of numerous in v...

When Semantics meets Phonetics: Acoustical studies of Second Occurrence Focus

A second occurrence (SO) focus is the semantic focus of a focus sensitive operator (e.g. only), but is a repeat of an earlier focused occurrence. SO foci are intonationally distinct from the original occurrence of the material, and are often claimed to lack any intonational marking, e.g. pitch accent (Partee 1999). This apparent dissociation of semantic and intonational focus is commonly used a...

When Science Meets Religion