Visual Detection of Biomolecules Using Concentration Dependent Induced Aggregation of Plasmonic Gold Nanoparticles

Significant advancement has occurred in the detection methods of solution-based analytes. High-pressure liquid chromatography, gas and other systems used for analyses are quite expensive. Therefore, there is a need new visible Here, we demonstrate that 3-aminopropyl triethoxysilane (APTES) could impact stability, optical, morphology gold nanoparticles (AuNps) colloidal solution. These impacts can be to create sensitive visual system. The strong APTES concentration on ultraviolet–visible absorption spectra solutions illustrated, which displays systematic extensive red shifts. presence denatured proteins within therapeutic drug product induce series adverse effects. This report describes fast, low cost, sensitive, user-friendly platform where plasmonic biosensing proteins. Artificially heat stressed ferritin, glutathione, insulin coupled AuNps exposed ATES upon denaturation protein or peptide, blue shifts observed absorbance AuNps/biomolecules, aminosilane serves as proof-of-concept fast in-solution method heat-stressed peptides.