Viable deletions of the M13 complementary strand origin
نویسندگان
چکیده
منابع مشابه
Deletion analysis of the cloned replication origin region from bacteriophage M13.
A cloned 270-nucleotide fragment from the origin region of the M13 duplex replicative form DNA confers an M13-dependent replication mechanism upon the plasmid vector pBR322. This M13 insert permits M13 helper-dependent replication of the hybrid plasmid in polA cells which are unable to replicate the pBR322 replicon alone. Using in vitro techniques, we have constructed several plasmids containin...
متن کاملIllegitimate recombination at the replication origin of bacteriophage M13.
Hybrids composed of phage M13 and plasmid pHV33 were used to study the formation of deletions in Escherichia coli. Eighty to ninety percent of the deletion endpoints were at the position of the nick introduced into the M13 replication origin by the phage gene II protein. This suggests the existence of a novel mechanism of illegitimate recombination.
متن کاملViable viruses with deletions in the left inverted terminal repeat define the adenovirus origin of DNA replication.
A series of human adenovirus type 2 genomes with deletions in the left inverted terminal repeat (ITR) have been constructed. Viral genomes that contained a minimum of 45 base pairs (bp) from the terminus of the genome were fully infectious and gave rise to progeny virus which maintained the deletion. In contrast, genomes containing 36 bp or less from the termini of the genome were not infectiou...
متن کاملInitiation signals for complementary strand DNA synthesis in the region of the replication origin of the Escherichia coli chromosome.
We have used an in vivo plasmid-phi X174 packaging system to detect replication initiation signals in the region of the replication origin (oriC) of the Escherichia coli chromosome. The results obtained are summarized as follows: (i) Neither within nor close to oriC effective signals for initiating complementary strand synthesis could be detected. We conclude that initiation mechanisms for lead...
متن کاملConversion of the M13 viral single strand to the double-stranded replicative forms by purified proteins.
Earlier studies showed that the Ml3 single-stranded circle is converted to duplex replicative forms (RF) in vivo and in uifro by a rifampicin-sensitive system that fails to act on +X174 DNA. In the absence of DNA polymerase I, the synthetic complementary strand produced by a crude cell extract contained a small gap at a unique position relative to the viral template strand. In these studies we ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1981
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.78.11.6784