Unravelling the Distribution of Secondary Metabolites in Olea europaea L.: Exhaustive Characterization of Eight Olive-Tree Derived Matrices by Complementary Platforms (LC-ESI/APCI-MS and GC-APCI-MS)

S-nitroso- and nitro- proteomes in the olive (Olea europaea L.) pollen. Predictive versus experimental data by nano-LC-MS

The data presented here are related to the research article entitled "Generation of nitric oxide by olive (Olea europaea L.) pollen during in vitro germination and assessment of the S-nitroso- and nitro-proteomes by computational predictive methods" doi:10.1016/j.niox.2017.06.005 (Jimenez-Quesada et al., 2017) [1]. Predicted cysteine S-nitrosylation and Tyr-nitration sites in proteins derived f...

Simultaneous quantification of opiates, cocaine, and metabolites in hair by LC-APCI-MS/MS.

A quantitative LC-APCI-MS/MS method for simultaneous measurement of opiates, cocaine, and metabolites in hair was developed and validated. Cocaine and opiates were extracted from pulverized hair via sonication in methanol at 37 degrees C for 3 h. Samples were cleaned up using solid-phase extraction. LC separation was achieved in 20 min with identification and quantification by selected reaction...

Chemical Analysis of Pesticides Using GC/MS, GC/MS/MS, and LC/MS/MS

There are well over 500 registered pesticides worldwide for use in agricultural regions and new agrochemicals are introduced to the marketplace continuously. This chapter deals with the chemical analysis methods for the main pesticide chemical classes that are most frequently analyzed with gas chromatography (GC) or liquid chromatography (LC) coupled to mass spectrometry (MS). GC amenable pesti...

Quantification of Glucuronide Metabolites in Biological Matrices by LC-MS/MS

For many drugs and xenobiotics glucuronidation is the major metabolic pathway of detoxification. There, a highly hydrophilic glucuronic acid is coupled to aliphatic / aromatic alcohols, thiols or carboxylic acids or even amines. Although the glucuronidation is usually considered a deactivation reaction, electrophilic acyl-glucuronides can be formed from a number of acidic drugs, such as diclofe...

Integrated Transcriptomics and Metabolomics Study of Retinoblastoma Using Agilent Microarrays and LC/MS/GC/MS Platforms