Threonine synthesis from homoserine as a selectable marker in mammalian cells
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چکیده
منابع مشابه
Threonine-insensitive Homoserine Dehydrogenase from Soybean
Amy C. Schroeder, Chuanmei Zhu, Srinivasa Rao Yanamadala, Rebecca E. Cahoon, Kiani A. J. Arkus, Leia Wachsstock, Jeremy Bleeke, Hari B. Krishnan, and Joseph M. Jez From the Department of Biology, Washington University, St. Louis, Missouri 63130, the Donald Danforth Plant Science Center, St. Louis, Missouri 63132, and the Plant Genetics Research Unit, United States Department of Agriculture-Agri...
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Four chick bioassays were conducted to evaluate the threonine (Thr) replacement value of l-homoserine (HS). Growth rate was increased (P < 0.05) by dietary addition of 800 mg l-HS/kg diet to a purified diet severely deficient in Thr or by the addition of 800 or 1000 mg of l-HS/kg diet to a corn-peanut meal diet distinctly deficient in Thr. The addition of an isomolar level of alpha-ketobutyrate...
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Homoserine kinase (HSK) produces O-phospho-l-homoserine (HserP) used by cystathionine gamma-synthase (CGS) for Met synthesis and threonine synthase (TS) for Thr synthesis. The effects of overexpressing Arabidopsis thaliana HSK, CGS, and Escherichia coli TS (eTS), each controlled by the 35S promoter, were compared. The results indicate that in Arabidopsis Hser supply is the major factor limiting...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1994
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2990637