The SOSS1 single-stranded DNA binding complex promotes DNA end resection in concert with Exo1
نویسندگان
چکیده
منابع مشابه
The SOSS1 single-stranded DNA binding complex promotes DNA end resection in concert with Exo1.
The human SSB homologue 1 (hSSB1) has been shown to facilitate homologous recombination and double-strand break signalling in human cells. Here, we compare the DNA-binding properties of the SOSS1 complex, containing SSB1, with Replication Protein A (RPA), the primary single-strand DNA (ssDNA) binding complex in eukaryotes. Ensemble and single-molecule approaches show that SOSS1 binds ssDNA with...
متن کاملPCNA promotes processive DNA end resection by Exo1
Exo1-mediated resection of DNA double-strand break ends generates 3' single-stranded DNA overhangs required for homology-based DNA repair and activation of the ATR-dependent checkpoint. Despite its critical importance in inducing the overall DNA damage response, the mechanisms and regulation of the Exo1 resection pathway remain incompletely understood. Here, we identify the ring-shaped DNA clam...
متن کاملSingle-molecule imaging reveals the mechanism of Exo1 regulation by single-stranded DNA binding proteins.
Exonuclease 1 (Exo1) is a 5'→3' exonuclease and 5'-flap endonuclease that plays a critical role in multiple eukaryotic DNA repair pathways. Exo1 processing at DNA nicks and double-strand breaks creates long stretches of single-stranded DNA, which are rapidly bound by replication protein A (RPA) and other single-stranded DNA binding proteins (SSBs). Here, we use single-molecule fluorescence imag...
متن کاملThe Human SRCAP Chromatin Remodeling Complex Promotes DNA-End Resection
BACKGROUND Repair of DNA double-strand breaks (DSBs) by homologous recombination requires 5'-3' resection of the DSB ends. In vertebrates, DSB resection is initiated by the collaborative action of CtIP and the MRE11-RAD50-NBS1 (MRN) complex. However, how this process occurs within the context of chromatin is still not well understood. RESULTS Here we identify the human SRCAP chromatin remodel...
متن کاملSingle-stranded DNA-binding Proteins
The emergence of DNA as the predominant hereditary material of life has necessitated the evolution of proteins whose sole function is the maintenance and care of these vital molecules. In a typical cell, there is a wide array of proteins with specialized functions in DNA metabolism that allow the cell to maintain and replicate its genome. In order to copy or make repairs to DNA, the double heli...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 2012
ISSN: 0261-4189,1460-2075
DOI: 10.1038/emboj.2012.314