The non-specific adenine DNA methyltransferase M.EcoGII

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The non-specific adenine DNA methyltransferase M.EcoGII

We describe the cloning, expression and characterization of the first truly non-specific adenine DNA methyltransferase, M.EcoGII. It is encoded in the genome of the pathogenic strain Escherichia coli O104:H4 C227-11, where it appears to reside on a cryptic prophage, but is not expressed. However, when the gene encoding M.EcoGII is expressed in vivo - using a high copy pRRS plasmid vector and a ...

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Dimeric restriction endonucleases and monomeric modification methyltransferases were long accepted as the structural paradigm for Type II restriction systems. Recent studies, however, have revealed an increasing number of apparently dimeric DNA methyltransferases. Our initial characterization of RsrI methyltransferase (M.RsrI) was consistent with the enzyme functioning as a monomer, but, subseq...

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 2017

ISSN: 0305-1048,1362-4962

DOI: 10.1093/nar/gkx1191