The major mRNA-associated protein YB-1 is a potent 5' cap-dependent mRNA stabilizer

Cap-dependent deadenylation of mRNA.

Poly(A) tail removal is often the initial and rate-limiting step in mRNA decay and is also responsible for translational silencing of maternal mRNAs during oocyte maturation and early development. Here we report that deadenylation in HeLa cell extracts and by a purified mammalian poly(A)-specific exoribonuclease, PARN (previously designated deadenylating nuclease, DAN), is stimulated by the pre...

Structural organization of mRNA complexes with major core mRNP protein YB-1.

YB-1 is a universal major protein of cytoplasmic mRNPs, a member of the family of multifunctional cold shock domain proteins (CSD proteins). Depending on its amount on mRNA, YB-1 stimulates or inhibits mRNA translation. In this study, we have analyzed complexes formed in vitro at various YB-1 to mRNA ratios, including those typical for polysomal (translatable) and free (untranslatable) mRNPs. W...

P-1: Leptin mRNA Is Present in Bovine EpididymalSpermatozoa

Background: The present study aimed to investigate the presence of leptin mRNA transcript in epididymal spermatozoa of Holstein cattle by RT-PCR analysis. Materials and Methods: To collect sperm from epididymis, the whole testes of three adult Holstein bulls were quickly removed after slaughtering. Samples were transferred to the laboratory on ice and subsequently the epididymal contents were c...

Heat shock impairs the interaction of cap-binding protein complex with 5' mRNA cap.

Cell-free protein synthesizing systems prepared from heat-shocked Ehrlich cells retain the inhibition of translation that is seen at the cellular level. Recently, we showed that a highly purified cap-binding protein complex composed of the p220 and p28 subunits of eukaryotic initiation factor 4F, in a 1:1 molar ratio, restores protein synthesis in these cell-free translation systems (Lamphear, ...

Interplay of YB-1 between Tubulin and mRNA