The Long Noncoding RNA Transcriptome of Dictyostelium discoideum Development
نویسندگان
چکیده
منابع مشابه
The Long Noncoding RNA Transcriptome of Dictyostelium discoideum Development
Dictyostelium discoideum live in the soil as single cells, engulfing bacteria and growing vegetatively. Upon starvation, tens of thousands of amoebae enter a developmental program that includes aggregation, multicellular differentiation, and sporulation. Major shifts across the protein-coding transcriptome accompany these developmental changes. However, no study has presented a global survey of...
متن کاملEvidence that noncoding RNA dutA is a multicopy suppressor of Dictyostelium discoideum STAT protein Dd-STATa.
Dd-STATa, a Dictyostelium discoideum homologue of metazoan STAT transcription factors, is necessary for culmination. We created a mutant strain with partial Dd-STATa activity and used it to screen for unlinked suppressor genes. We screened approximately 450,000 clones from a slug-stage cDNA library for their ability to rescue the culmination defect when overexpressed. There were 12 multicopy su...
متن کاملMacrocyst Development in Dictyostelium Discoideum
The early events of macrocyst development in Dictyostelium discoideum have been investigated using a new culturing system. When cells of opposite mating-types, HM1 and NC4, are cultured together at the appropriate temperature in the dark, giant cells appear, ingest the surrounding amoebae, and develop into macrocysts. Although these giant cells have been assumed to be the products of the fusion...
متن کاملdutA RNA functions as an untranslatable RNA in the development of Dictyostelium discoideum
dutA is a gene specifically expressed during the development of Dictyostelium discoideum. Toward understanding its possible role in development, we isolated and characterized the gene and its complete cDNA. We found that dutA is encoded by the nuclear genome as a single copy gene without introns. In addition, the following unique and interesting features of dutA RNA (1322 nt) emerged: (1) it ha...
متن کاملGenetics of early Dictyostelium discoideum development.
EARLY DEVELOPMENT OF DICTYOSTELIUM DISCOIDEUM.................................................... 29 GENETIC ANALYSIS.................................................. 30 MUTANTS ALTERED IN AGGREGATION.................................................. 32 Numbers of Genes .................................................. 32 Selection Procedures.................................................. ...
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ژورنال
عنوان ژورنال: G3 Genes|Genomes|Genetics
سال: 2017
ISSN: 2160-1836
DOI: 10.1534/g3.116.037150