The importance of system band broadening in modern size exclusion chromatography

Investigation and interpretation of band broadening in size exclusion chromatography.

Study of Band Broadening occurring in Size Exclusion Chromatography (SEC) is reported using very narrow PS standards obtained and characterised by Temperature Gradient Interaction Chromatography (TGIC). Chromatograms are fitted by Exponentially Modified Gaussian functions (EMG) and mapping of band broadening is obtained for different column sets. Interpretation of the skewing of the chromatogra...

the tragedy of modern man in arthur millers world

what miller wants is a theatre of heightened consciousness. he speaks of two passions in a man, the passion to "feel" and the passion "know". he belives that we can have more of the latter. he says: drama is akin to the other inventions of man in that it ought to help us know more and not merely to spend our feelings. the writing of the crucible shows us that he is trying to give more heightene...

Size Exclusion Chromatography of Polymers

In the characterization of polymers, size-exclusion chromatography (SEC) has become a standard technique for determining molar mass averages andmolar mass distributions (MMD) of polymers. The principle of SEC is easily understood: owing to limited accessability of the pore volume within the particles of the column packing, polymer molecules are separated according to their hydrodynamic volumes,...

Liposome retention in size exclusion chromatography

BACKGROUND Size exclusion chromatography is the method of choice for separating free from liposome-encapsulated molecules. However, if the column is not presaturated with lipids this type of chromatography causes a significant loss of lipid material. To date, the mechanism of lipid retention is poorly understood. It has been speculated that lipid binds to the column material or the entire lipos...

Measurement of Albuminuria with Size-exclusion Chromatography