The effect of a seed coating with Origanum vulgare essential oil on Clavibacter michiganensis subsp. michiganensis

The study aimed to evaluate the efficacy of a seed film coating with Origanum vulgare Linnaeus essential oil (EO) against Clavibacter michiganensis subsp. (Cmm) on tomatoes. Tomato seeds (cv. Rio Grande) coated different doses EO derived from O. were inoculated Cmm (1.8 × 108 CFU/mL). vulgare's showed remarkable inhibition effect growth. was determined based parameters, such as zone and bacterial population in seed. GC-MS analysis that carvacrol is major component (at 74.05%), which may inhibit Later, we have expanded our studies determine inhibitory EO's mode action pathogenic bacteria molecular docking protein-ligand interaction. results has strong interaction expansin protein (PDB 4JJO) qPCR analyses confirmed treatment Cmm. This original approach prominent potential prevent transmission for quality world, suggesting method paving way disease management.