The Determination of Calcium in Biological Fluids by Flame Photometry
نویسندگان
چکیده
منابع مشابه
The determination of calcium in biological fluids by flame photometry.
The use of flame photometers has greatly simplified and expedited the estimation of sodium and potassium. Calcium is one of the elements which it is possible to determine by flame photometry, but little advantage has been taken of this fact in biological work for a number of reasons. The concentration of calcium in extracellular fluids is usually less than that of potassium ; a given quantity o...
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A method is described for the measurement of magnesium in biological materials and has been applied to the Unicam flame spectrophotometer SP 900. Samples were diluted in 10 mM strontium ethylenedinitrolo tetraacetate (EDTASr), 10 mM potassium carbonate, giving final magnesium concentrations of about 0.1 to 0.2 mM. The EDTASr almost eliminated the depression of magnesium emission by interfering ...
متن کاملA new principle applied to the determination of calcium in biological materials by flame photometry.
The effect of magnesium sulphate in releasing calcium emission from interference by phosphate and sulphate has been investigated. Samples were diluted in 10 mM MgSO(4), 2 mM NaCl, giving final calcium concentrations of about 0.05 to 0.10 mM. In this diluent, galvanometer readings were proportional to calcium concentrations up to 0.4 mM. The magnesium sulphate released calcium emission from depr...
متن کاملCalcium determination by flame photometry; methods for serum, urine, and other fluids.
The convenience and accuracy of flame photometric determinations of sodium and potassium in biological fluids (1, 2) have suggested the desirability of extending these advantages to determinations of calcium. Since calcium emission lines are much less intense than those of sodium and potassium (3), the samples of diluted serum (1: 50) customarily used for sodium and potassium determinat.ions ar...
متن کاملThe application of flame photometry to sodium and potassium determinations in biological fluids.
The need for a rapid, accurate method to determine quantitatively Na and K present together in biological fluids has been met by the procedures to be described. The necessity of separating these elements prior to their determination by the various chemical methods usually employed has led to procedures which are often prohibitively tedious and time-consuming. The recent development of flame pho...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1953
ISSN: 0021-9746
DOI: 10.1136/jcp.6.4.286